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. 2014 Feb 7;4:4032. doi: 10.1038/srep04032

Figure 1. Difference and similarity in circadian phase of clock gene expression between five mouse peripheral tissues.

Figure 1

Circadian expression of nine clock genes in five mouse peripheral tissues was measured by real-time PCR. Relative levels of mRNA were normalized to the corresponding 18S-rRNA levels. The peak times calculated by cosine curve-fitting were compared. Each value represents the average of three independent RT-PCR experiments. (A) Upper panel: Phase comparison among the five mouse peripheral tissues for the nine clock genes. Lower panel: To determine the relative phase of these peripheral clocks, circadian phase relative to the liver was calculated for each clock gene, and these phase intervals were then averaged. (B) Upper panel: Phase relations among the nine clock genes in the five mouse peripheral tissues. Lower panel: The circadian phases of the nine clock genes relative to that of Per3 were calculated for each peripheral tissue, and these phase differences were then averaged.