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. Author manuscript; available in PMC: 2014 Dec 26.
Published in final edited form as: Oncogene. 2013 Aug 5;33(26):3422–3431. doi: 10.1038/onc.2013.308

Figure 1. Silencing MUC1-C downregulates HER2 activation and colony formation.

Figure 1

A. Lysates from SKBR3 and BT474 cells were immunoblotted with the indicated antibodies. B. Lysates from SKBR3 (left) and BT474 (right) cells were precipitated with anti-MUC1-C or a control IgG. The precipitates were immunoblotted with the indicated antibodies. C. SKBR3 (left) and BT474 (right) cells were infected with lentiviruses to stably express a control shRNA (CshRNA) or a MUC1shRNA. Lysates were immunoblotted with the indicated antibodies. D. The indicated SKBR3 (left) and BT474 (right) cells were seeded at 5 × 104 cells/well. The results (mean±SD of three replicates) are expressed as the cell number on day 4. E. The indicated SKBR3 (left) and BT474 (right) cells were seeded at 2000 and 1000 cells/well, respectively, and incubated for 12 d. Colony number is expressed as the mean±SD of three replicates.