Figure 1.

Transwell migration assay reveals serotonin (5-HT) induction of PC12 cell migration. (A) PC12 cells were spread onto the upper side of transwell inserts to allow migration through the membrane. In the bottom well, Dulbecco’s modified Eagle’s medium with or without chemical cues, serotonin (1 μM) and/or nerve growth factor (NGF) (100 ng/ml) were applied. 5-HT and NGF significantly increased the migrating cells. (B) 5-HT induced migration in a dose-dependent manner. P-values were calculated by two-way or one-way ANOVA followed by Tukey’s HSD. ^*P<0.05; ^**P<0.01. Data are presented as mean ± standard error. HSD, honestly significant difference.