Table I.
Primers for reverse transcription-polymerase chain reaction.
| Primer | Sequence | Product size (bp) | Reaction cycles |
|---|---|---|---|
| TNF-α | Sense: 5′-CCGATGGGTTGTACCTTGTC-3′ Antisense: 5′-GGGCTGGGTAGAGAATGGAT-3′ |
352 | 32 |
| MCP-1 | Sense 5′-GGAGCATCCACGTGTTGGC-‘3 Antisense: 5′-GTAGGAGTGACCAGTGTGACAGT-3′ |
391 | 33 |
| IL-6 | Sense: 5′-GATGCTACCAAACTGGATATAATC-3′ Antisense: 5′-GGTCCTTAGCCACTCCTTCTGTG-3′ |
269 | 37 |
| IL-1β | Sense: 5′-GAAATGCCACCTTTTGACAGTG-3′ Antisense: 5′-GAAGGTCCACGGGAAAGACAC-3′ |
225 | 38 |
| β-actin | Sense: 5′-TTCTTTGCAGCTCCTTCGTTGCCG-3′ Antisense: 5′-TGGATGGCTACGTACATGGCTGGG-3′ |
458 | 32 |
TNF-α, tumor necrosis factor α; MCP-1, monocyte chemotactic protein-1; IL, interleukin.