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. 2013 Feb 14;1(3):379–382. doi: 10.3892/br.2013.65

Figure 3.

Figure 3

Metallothionein (MT) expression in HaCaT cells treated with HgCl2. (A) Total cellular protein (50 μg) from HaCaT cells treated with 0.25–1.5 μM of HgCl2 was assessed by western blot analysis for MT expression. C, untreated cells; 0.25, 0.5, 0.75, 1.0 and 1.5, cells treated with 0.25, 0.5, 0.75, 1.0 and 1.5 μM of HgCl2, respectively. Constitutive expression of β-actin in HaCaT cells demonstrated the overall protein and quality in cell lysates. (B) Quantification of the expression levels of MT in HaCaT cells treated with 0.25–1.5 μM of HgCl2 and in untreated cells.