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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Cell Signal. 2013 Nov 13;26(2):313–322. doi: 10.1016/j.cellsig.2013.11.009

Figure 2. Sequencing Coverage Map of Peptides Detected From Samples Subjected to Eccentric Contractions or the Control Condition.

Figure 2

Mouse TA muscles were transfected with FLAG-Raptor. At 7 days post transfection, the muscles were subjected to a bout of eccentric contractions (EC), or the control condition, and collected 40 min later. The samples were then subjected to immunoprecipitation for the FLAG tag and resolved on a SDS-PAGE gel. The FLAG-Raptor band was cut-out of the gel, digested with Trypsin and then submitted for LC/MS/MS analysis as detailed in the methods. The sequencing coverage and phosphorylated residues detected in the control and EC samples are depicted in the figure.