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. 2014 Jan;160(Pt 1):142–148. doi: 10.1099/mic.0.071779-0

Table 2. Plasmids and oligonucleotide primers used for constructs.

Plasmids* Genotype Primer pairs† SOEing primers† Restriction
pBMF985 pKSV7 mpl H226T 328/492, 489/493 328/489 EcoRI, PstI
pBMF1001 pKSV7 mpl H226F 328/499, 489/500 328/489 EcoRI, PstI
pBMF1002 pKSV7 mpl H226R 328/501, 489/502 328/489 EcoRI, PstI
pBMF1194 pKSV7 mpl H226F-FlagN-cat 328/499, 489/500 328/489 EcoRI, PstI
pBMF1234 pKSV7 mpl H226R-FlagN-cat 328/487, 387/488 328/387 EcoRI, PstI
*

Plasmids pBMF1001 through pBMF1194 carry a silent mutation at mpl V227.

†

Primer sequences are listed in Table 3.