Skip to main content
. 2013 Dec 24;12(1):17–35. doi: 10.3390/md12010017

Figure 2.

Figure 2

Effects of synthetic 6-bromoisatin on HT29 cells: Cell viability (a), LDH release (b) and caspase-3/7 activity (c). LDH release was measured by fluorescence at 535EX/590EM and caspase-3/7 activity was measured at full light on a luminescence plate reader. The positive controls are lysis buffer (5 µL/well) for the LDH assay and staurosporine (Str) (5 µM) for apoptosis. A final concentration of 1% DMSO was used in all control and treated cells. The results are mean for three independent repeat assays (n = 3) each performed in triplicate. Significant differences between each group and the DMSO control are shown as p ≤ 0.05 (*) and p ≤ 0.001 (***).