Figure 5.
AD0157 inhibits angiogenesis in vivo. (A) Chorioallantoic membrane assay (CAM). The methylcellulose disc containing the substance vehicle alone and the methylcellulose disc containing 0.5, one and 5 nmol of AD0157. Circles show the locations of the methylcellulose discs (bar = 1 mm); (B) Inhibition of the zebrafish neovascularization by AD0157. Transgenic TG(fli1:EGFP)y1 zebrafish embryos that show green fluorescent protein (GFP) expression in endothelial cells were incubated without or with 10 µM AD0157 (bars represent 50 µm).
