Figure 2.

Inhibition of NS3 helicase-catalyzed RNA unwinding activity by hal3 and suvanine. (A,B) Inhibition curves of hal3 and suvanine generated using a fluorescence helicase assay. The NS3 helicase activities of samples containing inhibitor were calculated relative to control samples containing DMSO vehicle rather than inhibitor. The data are presented as mean ± standard deviation of three replicates; (C,D) Gel images representing the inhibitory effects of hal3 and suvanine in a gel-based helicase assay. Fluorescence-labeled ssRNA and dsRNA were applied to lanes 1 and 2, respectively. The dsRNA was incubated with NS3 in the presence of increasing concentrations of inhibitor (lanes 3–7, 0–100 µM). Lane 8 shows the control reaction in the absence of NS3.