Table 3.
Expression levels of genes involved in ergosterol biosynthesis
| ORF | Gene |
MA-R4 xylose/glucose (C1) |
Glucose MA-R4/MA-B4 (C4) |
Description | ||
|---|---|---|---|---|---|---|
| (C1-1) | (C1-2) | (C4-1) | (C4-2) | |||
| YPL028W |
ERG10 |
-9.74 |
-11.19 |
4.26 |
3.00 |
Acetyl-CoA C-acetyltransferase |
| YML126C |
ERG13 |
-9.74 |
-11.19 |
4.03 |
3.65 |
3-Hydroxy-3-methylglutaryl-CoA synthase |
| YML075C |
HMG1 |
-6.92 |
-7.35 |
6.03 |
6.54 |
3-Hydroxy-3-methylglutaryl-CoA reductase |
| YMR208W |
ERG12 |
-3.62 |
-4.78 |
3.76 |
3.87 |
Mevalonate kinase |
| YMR220W |
ERG8 |
-4.50 |
-3.45 |
2.11 |
2.28 |
Phosphomevalonate kinase |
| YNR043W |
MVD1 |
-9.39 |
-10.02 |
2.95 |
3.52 |
Mevalonate pyrophosphate decarboxylase |
| YPL117C |
IDI1 |
-2.85 |
-3.72 |
2.70 |
2.54 |
Isopentenyl diphosphate isomerase |
| YJL167W |
ERG20 |
-6.65 |
-5.63 |
3.94 |
2.73 |
Farnesyl pyrophosphate synthetase |
| YHR190W |
ERG1 |
-14.58 |
-9.67 |
6.22 |
3.79 |
Squalene epoxidase |
| YHR072W |
ERG7 |
-5.16 |
-5.59 |
2.36 |
3.24 |
Lanosterol synthase |
| YHR007C |
ERG11 |
-11.73 |
-9.61 |
6.15 |
4.27 |
Lanosterol 14-α-demethylase |
| YGR060W |
ERG25 |
-6.56 |
-7.09 |
6.17 |
5.99 |
C-4 methyl sterol oxidase |
| YGL001C |
ERG26 |
-3.79 |
-3.71 |
4.95 |
4.28 |
C-3 sterol dehydrogenase |
| YML008C |
ERG6 |
-4.50 |
-4.41 |
2.72 |
2.47 |
Delta-24-sterol C-methyltransferase |
| YMR202W |
ERG2 |
-21.42 |
-14.46 |
4.95 |
3.73 |
C-8 sterol isomerase |
| YLR056W |
ERG3 |
-8.00 |
-9.96 |
4.17 |
4.34 |
C-5 sterol desaturase |
| YMR015C | ERG5 | -4.08 | -4.95 | 4.55 | 6.70 | C-22 sterol desaturase |
Relevant genes for which the MA-R4 xylose/glucose ratio (C1) was lower than two, and the glucose MA-R4/MA-B4 ratio (C4) was higher than two, were selected. The DNA microarray analysis was repeated twice (Values shown in C1-2 and C4-2 are from dye-swap experiments).