Table 6.
Expression levels of genes with significantly changed in xylose metabolism relative to glucose metabolism
| ORF | Gene |
MA-R4 xylose/glucose (C1) |
MA-B4 xylose/glucose (C2) |
Description | ||
|---|---|---|---|---|---|---|
| (C1-1) | (C1-2) | (C2-1) | (C2-2) | |||
| Respiratory metabolism |
|
|
|
|
|
|
| YBL045C |
COR1 |
2.37 |
2.73 |
3.00 |
2.75 |
Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex) |
| Q0045 |
COX1 |
2.17 |
2.55 |
2.25 |
2.50 |
Subunit I of cytochrome c oxidase |
| YIL111W |
COX5B |
7.20 |
11.74 |
2.87 |
2.62 |
Subunit Vb of cytochrome c oxidase |
| YMR256C |
COX7 |
2.06 |
2.38 |
3.87 |
3.95 |
Subunit VII of cytochrome c oxidase |
| YGL191W |
COX13 |
2.15 |
2.89 |
4.21 |
5.33 |
Subunit VIa of cytochrome c oxidase |
| YDR231C |
COX20 |
4.97 |
4.39 |
4.61 |
4.94 |
Mitochondrial inner membrane protein |
| YJL166W |
QCR8 |
2.66 |
3.36 |
3.87 |
5.50 |
Subunit 8 of ubiquinol cytochrome c reductase complex |
| YAL039C |
CYC3 |
2.31 |
2.11 |
5.63 |
6.08 |
Cytochrome c heme lyase (holocytochrome c synthase) |
| YEL039C |
CYC7 |
7.40 |
8.71 |
31.54 |
37.06 |
Cytochrome c isoform 2 |
| YPL159C |
PET20 |
2.54 |
2.98 |
2.13 |
2.80 |
Mitochondrial protein |
| YLR154W-C |
TAR1 |
2.25 |
4.70 |
2.32 |
4.56 |
Mitochondrial protein |
| Hexose transporters |
|
|
|
|
|
|
| YHR094C |
HXT1 |
-30.45 |
-21.51 |
-56.36 |
-46.31 |
Low-affinity glucose transporter |
| YDR345C |
HXT3 |
-54.60 |
-20.87 |
-83.61 |
-32.66 |
Low-affinity glucose transporter |
| YHR096C |
HXT5 |
22.20 |
48.69 |
22.78 |
44.86 |
Hexose transporter with moderate affinity for glucose |
| Galactose metabolism |
|
|
|
|
|
|
| YBR020W |
GAL1 |
19.10 |
32.47 |
3.71 |
5.48 |
Galactokinase |
| YDR009W |
GAL3 |
4.20 |
4.91 |
6.88 |
9.85 |
Transcriptional regulator involved in activation of the GAL genes in response to galactose |
| YPL248C |
GAL4 |
3.44 |
4.04 |
9.38 |
7.75 |
DNA-binding transcription factor required for the activation of the GAL genes in response to galactose |
| YBR018C |
GAL7 |
7.05 |
13.98 |
5.29 |
10.70 |
Galactose-1-phosphate uridyl transferase |
| YBR019C |
GAL10 |
45.93 |
203.38 |
16.32 |
14.96 |
UDP-glucose-4-epimerase |
| Spore wall metabolism |
|
|
|
|
|
|
| YHR139C |
SPS100 |
53.16 |
93.57 |
12.99 |
18.00 |
Protein required for spore wall maturation |
| YDR403W |
DIT1 |
4.77 |
8.66 |
4.68 |
6.11 |
Sporulation-specific enzyme required for spore wall maturation |
| YDR402C |
DIT2 |
3.86 |
2.59 |
6.18 |
3.33 |
N-formyltyrosine oxidase |
| YGR032W |
GSC2 |
10.25 |
15.12 |
9.91 |
17.89 |
Catalytic subunit of 1,3-beta-glucan synthase |
| YMR306W |
FKS3 |
8.57 |
9.05 |
10.66 |
7.54 |
Protein involved in spore wall assembly |
| YDL239C |
ADY3 |
2.89 |
3.82 |
2.31 |
2.26 |
Protein required for spore wall formation |
| Ammonia secretion |
|
|
|
|
|
|
| YNR002C |
ATO2 |
8.78 |
36.19 |
9.57 |
13.26 |
Putative transmembrane protein involved in export of ammonia |
| Trehalose metabolism |
|
|
|
|
|
|
| YBR126C |
TPS1 |
4.95 |
6.86 |
4.75 |
7.17 |
Synthase subunit of trehalose-6-P synthase/phosphatase complex |
| YDR074W |
TPS2 |
5.27 |
4.61 |
4.55 |
4.72 |
Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex |
| YML100W | TSL1 | 17.88 | 18.11 | 8.01 | 6.82 | Large subunit of trehalose 6-phosphate synthase/phosphatase complex |
Relevant genes for which the MA-R4 xylose/glucose ratio (C1) was lower than two, and the MA-B4 xylose/glucose ratio (C2) was higher than two, were selected. The DNA microarray analysis was repeated twice (Values shown in C1-2 and C2-2 are from dye-swap experiments).