Table 1.
Strains and plasmids used in this work
| Strain | Genotype | Comments | Source |
|---|---|---|---|
| HCB1660 | fliC::Tn5 (KanR) | ‘wild type’ Contains plasmid pBAD33-fliCS219C | (Turner et al., 2010) Gift of H Berg |
| PM87 | cheBYZ::FRT, fliC::Tn5 (KanR) | ‘CheY*’ Contains plasmids pMS164 and pPM5 | This study |
| RP437 | Wild-type for chemotaxis | (Parkinson and Houts, 1982) | |
| SK109 | cheBYZ::Cm | This study | |
| SK110 | cheBYZ::FRT | This study | |
| SK112 | cheBYZ::FRT, fliC::Tn5 (KanR) | This study | |
| Plasmids | |||
| pBAD33 fliCS219C | fliCS219C under ParaBAD promoter, CmR, p15a origin | Expresses mutant version of FliC for fluorescent labeling | (Turner et al., 2010) Gift of H Berg |
| pPM5 | fliCS219C under ParaBAD promoter, AmpR, colE1 origin | Expresses mutant version of FliC for fluorescent labeling | This study |
| pMS164 | cheYD13K under PlacOP promoter, CmR, pSC101 origin | Expresses constitutively active version of CheY | (Alon et al., 1998) Gift of P Cluzel |
| pDK46 | Helper plasmid | (Datsenko and Wanner, 2000) | |
| pKD3 | Template for CmR cassette | (Datsenko and Wanner, 2000) | |
| pCP20 | Helper plasmid | (Cherepanov and Wackernagel, 1995) |