Table 2.
Primers used in this work
| Primer | Sequence | Comments |
|---|---|---|
| SK140F | TGCGTGGTCAGACGGTGTATGCGCTAAGTAAGGATTAACG GTGTAGGCTGGAGCTGCTTC | cheBYZ deletion forward |
| SK140R | GCCTGATATGACGTGGTCACGCCACATCAGGCAATACAAA CATATGAATATCCTCCTTAG | cheBYZ deletion reverse |
| SK141F | CCTTAAACCCGACGGATTGC | cheBYZ deletion check forward |
| SK141R | TTGCTGCCACACATCAAGC | cheBYZ deletion check reverse |
| SK163F | AGGGTTATTGTCTCATGAGC | pZE11 sequencing forward |
| SK163R | GTTTTATTTGATGCCTCTAG | pZE11 sequencing reverse |
| PM7F | GGG GACGTC ATCGATGCATAATGTGCCTG | amplify ParaBAD fliCS219C forward |
| PM7R | GGG GTCGAC TTAACCCTGCAGC | amplify ParaBAD fliCS219C reverse |