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. 2014 Jan 7;6(1):137–150. doi: 10.3390/v6010137

Figure 1.

Figure 1

Schematic presentation of the Class III Biosafety Cabinet setup at the IRF-Frederick. The Class III biosafety cabinet (BSC) is a hermetically sealed stainless steel cabinet containing an ABSL-4 environment under negative pressure (currently in static state for easy viewing) within an ABSL-4 cabinet laboratory. Materials can be introduced into the BSC by staff working in the ABSL-4 cabinet laboratory through an under-cabinet-mounted stainless steel tank containing the disinfectant Micro-Chem Plus® (commonly referred to as a “dunk tank” in ABSL-4 or BSL-4 settings). Because the BSC is built into the wall separating the cabinet laboratory from an ABSL-4 suit laboratory, materials, animals, and viral pathogens can also be moved into the BSC from the ABSL-4 suit laboratory side using a transport cart and a Rapid Transfer Port (RTP). The contents within the BSC can be manipulated from the outside by researchers wearing various types of synthetic rubber gloves, specifically neoprene/chlorosulphonated polyethylene. Contents, excluding infectious samples, are removed from the BSC after sterilization through a double-door autoclave or disinfection via the dunk tank.