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. 2014 Jan 7;6(1):137–150. doi: 10.3390/v6010137

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Interlocking double-door autoclave attached to the Class III BSC. Used contents and waste generated during an experiment inside the BSC must be sterilized after use. A researcher is selecting a pre-programmed autoclave cycle to ensure the contents within the autoclave chamber are noninfectious when the outer door is eventually opened. The door located nearest to the researcher cannot be opened until a full sterilization cycle has been completed. Biological indicators inside the autoclave chamber will be analyzed to determine agent inactivation after the sterilization process.