Table 2.

Oligonucleotides used for HCMV BAC mutagenesis.

Primer name	Nucleotide sequence
UL77-ko.for	5'-acgatgccatcacgggacccgccgccgccccgtctgacgtggaaaagtgccacctgcagat-3'
UL77-ko.rev	5'-ccgaggacgttcgccctttatgcagcgagcgacacgtggtgcaggaacacttaacggctga-3'
UL104-ko.for	5'-gctgtcgtcgtaggcggcggccacgatctcgccgaaggagagaaaagtgccacctgcagat-3'
UL104-ko.rev	5'-gtgctgggcggcctccgcgacattttatatcagtacgccgcaggaacacttaacggctga-3'
UL44-ko.for	5'-gggaccgcgccgtgcgcgcgttcccaggcacgcggcccgcgaaaagtgccacctgcagat-3'
UL44-ko.rev	5'-tcgctcgctcgcgcccgctccttagtcgagacttgcacgctcaggaacacttaacggctga-3'
UL93-ko.for	5'-agtcgaactataccgggcgttggacgcttatcgggcgtagtgactgattcgcgccgtgcgcaa-ggatgacgacgataag-3'
UL93-ko.rev	5'-agtacagcgtagatctcgtcgcgcacggcgcgaatcagtcactacgcccgataagcgtccagcc-agtgttacaa­ccaatt-3'