Table 2.
Specific activities of different xylose isomerase and xylulokinase
| Specific activity (U mg−1 total protein) | ||||||||
|---|---|---|---|---|---|---|---|---|
| WT(pEKEx3-x) |
||||||||
| – | xylAEc | xylABs | xylAMs | xylAXc | xylAXc-xylBEc | xylAXc-xylBBs | xylAXc-xylBCg | |
| Xylose isomerase | < 0.005 | 0.095 ± 0.010 | 0.023 ± 0.003 | 0.033 ± 0.007 | 0.090 ± 0.008 | 0.062 ± 0.004 | 0.026 ± 0.003 | 0.077 ± 0.010 |
| Xylulokinase | 0.013 ± 0.005 | 0.020 ± 0.000 | 0.024 ± 0.000 | 0.019 ± 0.000 | 0.021 ± 0.000 | 0.541 ± 0.063 | 0.020 ± 0.004 | 0.468 ± 0.018 |
All tests were carried out with crude extracts at 30°C.