Figure 3.

Application of eYFP (grey) and destabilized eYFP-ASV (black) for dynamic gene expression analysis of gntK in C. glutamicum. Cells were inoculated to an OD₆₀₀ of 1 in 750 μl of CGXII minimal medium containing either 100 mM gluconate (A) or 50 mM glucose and gluconate (B) in 48-well microtiter plates in the BioLector cultivation system (m2p labs, Germany). The final backscatter corresponds to a maximum OD₆₀₀ of 35 and 38 for growth on 100 mM gluconate and 50 mM glucose plus gluconate, respectively. For pre-cultures, cells were cultivated in CGXII with 100 mM glucose. Growth (dashed line) and fluorescence (solid line) were recorded in 15 min intervals (for details see Fig. 1).