Table 2.

Overview of SsrA tag variants.

SsrA-tag	Amino acid sequence	Half-life (min)a	Signal intensity (%)b
E. c. LAA-tag native	AANDENYALAA	n.d.	n.d.
C. g. LAA-tag native	AAEKSQRDYALAA	n.d.	n.d.
C. g. LVA-tag variation	AAEKSQRDYALVA	n.d.	n.d.
C. g. ASV-tag variation	AAEKSQRDYAASV	22 ± 4	46
C. g. AAV-tag variation	AAEKSQRDYAAAV	8 ± 3	20

^aProtein half-lives were calculated from Western blot analysis (Fig. 1C) corresponding to the decay law . Given values represent the average values with standard deviation of three independent experiments. For indicated constructs rapid degradation occurred and half-lives could not be determined (n.d.).

^bSignal intensity relative to native eYFP at the time of measurement.