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. Author manuscript; available in PMC: 2014 Feb 7.
Published in final edited form as: Biotechnol Appl Biochem. 2012 Oct 10;59(5):353–366. doi: 10.1002/bab.1034

Fig. 5.

Fig. 5

Neutralization of IN function in one-cycle replication assay. Values represent the percentages of one cycle replication in HeLa CD4 LTR-β-Gal cells relative to the value obtained for the wild-type virus. Cells were transfected with plasmids encoding anti-IN antibody fragments as described in Materials and methods. The ability of anti-IN antibody fragments to inhibit a single round of replication was measured by quantification of the β-galactosidase activity in cell lysates using a colorimetric assay based on the cleavage of CPRG by β-galactosidase as described in Materials and methods. Anti-LANA1 scFv (BM10) was used as a control antibody. C + indicates HIV-1NL4-3 infection of control HeLa CD4 LTR-β-Gal cells; C− indicates uninfected HeLa CD4 LTR-β-Gal cells. Optical density at 570 nm was measured and data represent mean ± SEM (n = 3).