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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2013 Oct 16;70(12):819–836. doi: 10.1002/cm.21146

Fig. 9. FRET Efficiencies (E%'s) plotted as a function of donor-to-acceptor (D:A) ratio for cells co-expressing CFP-Rac1 or CFP-Cdc42 plus Teal-N-WASP, Venus-IQGAP1, and mRFP-Rac1 or mRFP1-Cdc42Cherry-actin, and exhibiting 3-color FRET.

Fig. 9

Each data point represents a single ROI at either the cell periphery or cell-cell boundary. Data for cell expressing unfused CFP + Venus + mCherry are also shown. Least squares linear regression was used to generate the straight line plot for each data set, and p-values derived from T-tests for each pair of data sets are shown in Table S1, Supplementary Information. Least squares linear regression was used to generate the straight line plot for each data set.