Table 3.
Main Properties of the Major Toxins of C. perfringens (Modified from Sakurai, 1995)
| CPA | CPB | ETX | ITX | PFO | CPE | CPB2 | |
|---|---|---|---|---|---|---|---|
| Molecular weight | 42.528 kDa | 35 kDa | 34 kDa | Ia: 47,500 Ib: 71,500 |
54 kDa | 35 kDa | 28 kDa |
| LD 50* (mice) | 3 μg | 1.87 μg/ip; 30 ng-50 μg/iv | 100 μg | Ia: 620 μg Ib: 940 μg |
Not reported | 50 μg | 0.3 μg |
| Biological activity | Lethal Necrotizing Hemolytic Contraction of smooth muscle |
Dermonecrotic Edema, Enterotoxic, Cytotoxic | Dermonecrotic Lethal Edema Contraction of smooth muscle |
Lethal Necrotizing |
Lethal, Necrotizi ng | Erythema Lethal Enterotoxic |
Dermonecrotic Edema, Enterotoxic, Cytotoxic |
| Thermostability | Stable | Inactivated at 60°C (Sakurai and Duncan, 1978), ~2 month at −80°C | Up to 1 week at room temperature, several months at −20C years at −80 C | Ia. Stable Ib: Labile |
Unknown | Inactivated at 60°C <1 week at RT ~1 month at − 20°C, years lyophilized |
Unknown |
| Reaction to Trypsin | Susceptible | Sensitive (inactivated) | Activated (resistant) | Activated (resistant) | Susceptible | Activated (resistant) | Sensitive (inactivated) |
*
per kg of mouse
ip: intraperitoneal injection
iv: intravenous injection