Figure 2. Expression of PSA-NCAM and GFAP in spheroids and differentiation into immature oligodendrocytes and astrocytes under different growth conditions.

(A) Spheroids were gently dissociated and plated on to PDL-coated chamber slides for PSA-NCAM and GFAP immunostaining. (B) Spheroids were dissociated into single cells, plated for 6 h with 2% serum and grown in an atmospheric incubator. Media was changed to N2B2 with 0.5% serum for 96 h. Cells were then stained for O4, followed by fixation and then for GFAP and DAPI. Data represent averages±S.E.M. from three independent experiments. * denote significant differences in (A) versus Pro-N/B104. n.s. denotes no statistical significance. For comparisons in both (A) and (B), P<0.05 using ANOVA followed by Fisher's PLSD post-hoc test.