Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 7;6(2):e00137. doi: 10.1042/AN20120041

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Spheroids were cultured with 10 ng/ml PDGF-AA, differentiated for 3 days in either 0.5% FBS or 20% FBS then stained for oligodendrocytes (Rmab) (A1, B1, C1), O-2A lineage (A2B5) (A2, B2, C2), and astrocytes (GFAP) (A3, B3, C3). The pseudocolor images show an overlay of Rmab (magenta), A2B5 (green), GFAP (red), and DAPI (blue) (A4, B4, C4). When differentiated in 0.5% FBS and 10 ng/ml BMP-4, a single spheroid produced neurons (TUJ1), type 1 astrocytes (A2B5−/GFAP+), type 2 astrocytes (A2B5+/GFAP+) and oligodendrocytes (Rmab) (D). Examples of the four cell types from a single spheroid neuron (D1), type 1 astrocyte (D2), type 2 astrocyte (D3) and oligodendrocyte (D4) within the spheroid. Scale bar represents 20 μm.