Skip to main content
. Author manuscript; available in PMC: 2014 Feb 7.
Published in final edited form as: Biochim Biophys Acta. 2011 Dec 13;1818(3):703–717. doi: 10.1016/j.bbamem.2011.12.005

Figure 4.

Figure 4

Demonstration that 8 TMS TSUP family members arose by intragenic duplication of a primordial 4 TMS encoding genetic element. (A) GAP alignment of TMSs 1–4 of TSUP Tko1 (Thermococcus kodakarensis; gi# 57640914) with TMSs 5–8 of the same protein. Initial identification of repeat units was done using the IC program. The GAP program was run with default settings and 500 random shuffles. Residue identities are signified by vertical lines, while close and more distant similarities are signified by colons or periods, respectively. The numbers at both ends of each line signify the positions of the residues in the proteins. TMS positions were predicted using the TMHMM 2.0 program for this and subsequent comparisons. A comparison score of 26 S.D. was obtained. (B) GAP alignment of TMSs 1–4 of TSUP Mch1 (Microcoleus chthonoplastes; gi# 224407624) with TMSs 5–8 of Mch1. A comparison score of 17 S.D. was obtained.