Table 1.
The 189 TSUP proteins included in this study. Proteins are listed clockwise starting from Cluster 1 in Fig. 1. Protein abbreviations, taxonomic origins, protein sizes (aas), gi numbers, organismal phyla, organismal domains, putative numbers of TMSs, and N-terminal orientation are included (see key below). N-terminal orientation results lacking asterisks signify agreement between programs. Average size and standard deviation values are provided for all clusters with two or more members. Additional average size and SD values are provided after removing the specified outliers.
| Abbreviation | Organism | Protein Size | GenBank No. | Phylum | Domain | No. of TMSs |
|---|---|---|---|---|---|---|
| Cluster 1 | ||||||
| Cmu1 | Cryptosporidium muris RN66 | 525 | 209881434 | Apicomplexa | Eukaryota | 11 |
| Cho1 | Cryptosporidium hominis TU502 | 518 | 67601741 | Apicomplexa | Eukaryota | 10 |
| Tpa2 | Theileria parva strain Muguga | 409 | 71033393 | Apicomplexa | Eukaryota | 9 |
| Ehi1 | Entamoeba histolytica | 460 | 67466247 | none | Eukaryota | 10 |
| Ddi1 | Dictyostelium discoideum AX4 | 549 | 66825573 | none | Eukaryota | 10 |
| Mbr2 | Monosiga brevicollis MX1 | 499 | 167525260 | Codonosigidae | Eukaryota | 8 |
| Tva1 | Trichomonas vaginalis G3 | 448 | 123437805 | Trichomonada | Eukaryota | 9 |
| Ath3 | Arabidopsis thaliana | 491 | 6554197 | Viridiplantae | Eukaryota | 9 |
| Ath5 | Arabidopsis thaliana | 431 | 2911082 | Viridiplantae | Eukaryota | 9 |
| Sbi3 | Sorghum bicolor | 473 | 242058941 | Viridiplantae | Eukaryota | 9 |
| Ppa1 | Physcomitrella patens subsp. patens | 405 | 168065030 | Viridiplantae | Eukaryota | 9 |
| Gma1 | Glycine max | 469 | 83853809 | Viridiplantae | Eukaryota | 9 |
| Psi1 | Picea sitchensis | 505 | 148906357 | Viridiplantae | Eukaryota | 10 |
| Sbi2 | Sorghum bicolor | 383 | 242044420 | Viridiplantae | Eukaryota | 7 |
| Mpu1 | Micromonas pusilla CCMP1545 | 461 | 226458924 | Viridiplantae | Eukaryota | 8 |
| Tps2 | Thalassiosira pseudonana CCMP1335 | 564 | 223992571 | Bacillariophyta | Eukaryota | 11 |
| Tps3 | Thalassiosira pseudonana CCMP1335 | 385 | 223998204 | Bacillariophyta | Eukaryota | 7 |
| Ptr2 | Phaeodactylum tricornutum CCAP 1055/1 | 644 | 219112381 | Bacillariophyta | Eukaryota | 11 |
| Pma6 | Perkinsus marinus ATCC 50983 | 385 | 239878631 | Perkinsidae | Eukaryota | 6 |
| Tgo3 | Toxoplasma gondii GT1 | 482 | 221485444 | Apicomplexa | Eukaryota | 10 |
| Tps1 | Thalassiosira pseudonana CCMP1335 | 522 | 224014684 | Bacillariophyta | Eukaryota | 9 |
| Tgo4 | Toxoplasma gondii VEG | 665 | 221505087 | Apicomplexa | Eukaryota | 11 |
| Bsa1 | Bodo saltans | 526 | 206598109 | Bodonidae | Eukaryota | 10 |
| Lma1 | Leishmania major strain Friedlin | 511 | 157873729 | Trypanosomatidae | Eukaryota | 10 |
| Mbr1 | Monosiga brevicollis MX1 | 512 | 167521960 | Codonosigidae | Eukaryota | 9 |
| Pte6 | Paramecium tetraurelia strain d4-2 | 491 | 145483119 | Oligohymenophorea | Eukaryota | 9 |
| Pte8 | Paramecium tetraurelia strain d4-2 | 473 | 145514235 | Oligohymenophorea | Eukaryota | 9 |
| Pte4 | Paramecium tetraurelia strain d4-2 | 424 | 145501808 | Oligohymenophorea | Eukaryota | 10 |
| Pte1 | Paramecium tetraurelia strain d4-2 | 441 | 145493138 | Oligohymenophorea | Eukaryota | 10 |
| Tth2 | Tetrahymena thermophila | 570 | 118348626 | Oligohymenophorea | Eukaryota | 9 |
| Pte7 | Paramecium tetraurelia strain d4-2 | 430 | 145531341 | Oligohymenophorea | Eukaryota | 10 |
| Tth1 | Tetrahymena thermophila | 505 | 146183328 | Oligohymenophorea | Eukaryota | 11 |
| Tth3 | Tetrahymena thermophila | 503 | 118395416 | Oligohymenophorea | Eukaryota | 10 |
| Tth4 | Tetrahymena thermophila | 1325 | 118401229 | Oligohymenophorea | Eukaryota | 8 |
| Pte5 | Paramecium tetraurelia strain d4-2 | 400 | 145528512 | Oligohymenophorea | Eukaryota | 10 |
| Pte2 | Paramecium tetraurelia strain d4-2 | 406 | 145538953 | Oligohymenophorea | Eukaryota | 9 |
| Osa2 | Oryza sativa Japonica Group | 465 | 222625716 | Viridiplantae | Eukaryota | 5 |
| Cre1 | Chlamydomonas reinhardtii | 929 | 159479540 | Viridiplantae | Eukaryota | 6 |
| Gla2 | Giardia lamblia ATCC 50803 | 748 | 159117352 | Hexamitidae | Eukaryota | 10 |
| Tgo2 | Toxoplasma gondii VEG | 299 | 221501858 | Apicomplexa | Eukaryota | 2 |
| Cre2 | Chlamydomonas reinhardtii | 1854 | 159469083 | Viridiplantae | Eukaryota | 9 |
| Pte3 | Paramecium tetraurelia strain d4-2 | 454 | 145493226 | Oligohymenophorea | Eukaryota | 7 |
| Tgo1 | Toxoplasma gondii GT1 | 1659 | 221487433 | Apicomplexa | Eukaryota | 8 |
| Average Size= 572 +/− 312 (all) | ||||||
| Average Size= 476 +/− 71 (w/out Tth4, Cre1, Gla2, Cre2, Tgo1) | ||||||
| Cluster 2 | ||||||
| Ssp3 | Sphingomonas sp. SKA58 | 259 | 94497264 | Alphaproteobacteria | Bacteria | 7 |
| Rsp4 | Ruegeria sp. TM1040 | 252 | 99082858 | Alphaproteobacteria | Bacteria | 5 |
| Pas1 | Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949 | 260 | 211638062 | Gammaproteobacteria | Bacteria | 7 |
| Vpa2 | Variovorax paradoxus S110 | 270 | 239813891 | Betaproteobacteria | Bacteria | 7 |
| Dno1 | Dichelobacter nodosus VCS1703A | 258 | 146329063 | Gammaproteobacteria | Bacteria | 6 |
| Sna1 | Stackebrandtia nassauensis DSM 44728 | 256 | 229865833 | Actinobacteria | Bacteria | 6 |
| Par1 | Psychrobacter arcticus 273-4 | 251 | 71066392 | Gammaproteobacteria | Bacteria | 5 |
| Hca1 | Helicobacter canadensis MIT 98-5491 | 250 | 224418685 | Epsilonproteobacteria | Bacteria | 7 |
| Eta1 | Edwardsiella tarda | 280 | 158512112 | Gammaproteobacteria | Bacteria | 6 |
| Msp4 | Marinomonas sp. MED121 | 258 | 87120732 | Gammaproteobacteria | Bacteria | 7 |
| Orf2 | gamma proteobacterium | 250 | 90416226 | Gammaproteobacteria | Bacteria | 6 |
| Cje1 | Campylobacter jejuni RM1221 | 254 | 57238492 | Epsilonproteobacteria | Bacteria | 7 |
| Orf5 | bacterium Ellin514 | 268 | 223939838 | Verrucomicrobia | Bacteria | 6 |
| Msp1 | Marinomonas sp. MED121 | 253 | 87118707 | Gammaproteobacteria | Bacteria | 8 |
| Ftu1 | Francisella tularensis subsp. holarctica FSC200 | 366 | 167010238 | Gammaproteobacteria | Bacteria | 8 |
| Avi1 | Azotobacter vinelandii DJ | 289 | 226943937 | Gammaproteobacteria | Bacteria | 7 |
| Psp4 | Psychromonas sp. CNPT3 | 274 | 90407709 | Gammaproteobacteria | Bacteria | 8 |
| Nar1 | Novosphingobium aromaticivorans DSM 12444 | 256 | 87200262 | Alphaproteobacteria | Bacteria | 6 |
| Ade1 | Anaeromyxobacter dehalogenans 2CP-C | 254 | 86157393 | Deltaproteobacteria | Bacteria | 7 |
| Dpi1 | Desulfovibrio piger ATCC 29098 | 259 | 212704568 | Deltaproteobacteria | Bacteria | 7 |
| Fva1 | Fusobacterium varium ATCC 27725 | 276 | 253583632 | Fusobacteria | Bacteria | 7 |
| Tps5 | Thermoanaerobacter pseudethanolicus ATCC 33223 | 253 | 167038325 | Firmicutes | Bacteria | 7 |
| Bmu1 | Brachyspira murdochii DSM 12563 | 255 | 227999578 | Spirochaetes | Bacteria | 6 |
| Vpa1 | Veillonella parvula DSM 2008 | 264 | 227372642 | Firmicutes | Bacteria | 8 |
| Ban1 | Bacillus anthracis str. A2012 | 263 | 65318350 | Firmicutes | Bacteria | 8 |
| Taf1 | Thermosipho africanus TCF52B | 254 | 217077973 | Thermotogae | Bacteria | 7 |
| Tde1 | Treponema denticola ATCC 35405 | 262 | 42525707 | Spirochaetes | Bacteria | 7 |
| Cbo6 | Clostridium bolteae | 251 | 160940895 | Firmicutes | Bacteria | 7 |
| Cac1 | Cloacamonas acidaminovorans | 257 | 218961280 | none | Bacteria | 7 |
| Psy1 | Pseudomonas syringae pv. oryzae | 258 | 237801487 | Gammaproteobacteria | Bacteria | 6 |
| Ttu1 | Teredinibacter turnerae T7901 | 256 | 237685094 | Gammaproteobacteria | Bacteria | 7 |
| Orf4 | uncultured marine bacterium 439 | 252 | 40062756 | none | Bacteria | 7 |
| Gbe1 | Granulibacter bethesdensis CGDNIH1 | 253 | 114328287 | Alphaproteobacteria | Bacteria | 7 |
| Lho1 | Laribacter hongkongensis HLHK9 | 310 | 226942144 | Betaproteobacteria | Bacteria | 6 |
| Rco1 | Ricinus communis | 301 | 223512929 | Viridiplantae | Eukaryota | 8 |
| Nmu1 | Neisseria mucosa ATCC 25996 | 256 | 225367635 | Betaproteobacteria | Bacteria | 7 |
| Sli1 | Spirosoma linguale DSM 74 | 254 | 229867512 | Bacteroidetes | Bacteria | 6 |
| Cps1 | Corynebacterium | 260 | 227490282 | Actinobacteria | Bacteria | 8 |
| pseudogenitalium ATCC 33035 | ||||||
| Jde1 | Jonesia denitrificans DSM 20603 | 269 | 227383462 | Actinobacteria | Bacteria | 7 |
| Pac1 | Propionibacterium acnes KPA171202 | 255 | 50842975 | Actinobacteria | Bacteria | 6 |
| Average Size= 264 +/− 21 (all) | ||||||
| Cluster 3 | ||||||
| Mka1 | Methanopyrus kandleri AV19 | 252 | 20093583 | Euryarchaeota | Archaea | 7 |
| Dde1 | Desulfovibrio desulfuricans | 379 | 220904085 | Deltaproteobacteria | Bacteria | 9 |
| Dth2 | Desulfonatronospira thiodismutans ASO3-1 | 254 | 225199785 | Deltaproteobacteria | Bacteria | 7 |
| Rca1 | Roseiflexus castenholzii | 251 | 156743559 | Chloroflexi | Bacteria | 8 |
| Orf6 | uncultured bacterium | 654 | 239787713 | none | Bacteria | 9 |
| Average Size= 358 +/− 174 (all) | ||||||
| Average Size= 252 +/− 2 (w/out Orf6, Dde1) | ||||||
| Cluster 4 | ||||||
| Iho1 | Ignicoccus hospitalis KIN4/I | 240 | 156936864 | Crenarchaeota | Archaea | 7 |
| Bsp1 | Beggiatoa sp. PS | 787 | 153869281 Gammaproteobacteria | Bacteria | 10 | |
| Cbu3 | Coxiella burnetii Dugway 5J108-111 | 274 | 209364180 Gammaproteobacteria | Bacteria | 8 | |
| Cgl1 | Chryseobacterium gleum ATCC 35910 | 505 | 227369714 | Bacteroidetes | Bacteria | 8 |
| Gla1 | Giardia lamblia ATCC 50803 | 520 | 159115095 | Hexamitidae | Eukaryota | 10 |
| Min1 | Methylacidiphilum infernorum V4 | 267 | 189218632 | Verrucomicrobia | Bacteria | 8 |
| Average Size= 432 +/− 214 (all) | ||||||
| Average Size= 260 +/− 18 (w/out Bsp1, Cgl1, Gla1) | ||||||
| Cluster 5 | ||||||
| She1 | Slackia heliotrinireducens DSM 20476 | 277 | 229879562 | Actinobacteria | Bacteria | 8 |
| Ele1 | Eggerthella lenta DSM 2243 | 307 | 227411437 | Actinobacteria | Bacteria | 8 |
| Rxy1 | Rubrobacter xylanophilus DSM 9941 | 267 | 108803101 | Actinobacteria | Bacteria | 8 |
| Sfu1 | Syntrophobacter fumaroxidans MPOB | 269 | 116750841 | Deltaproteobacteria | Bacteria | 7 |
| Ptr3 | Phaeodactylum tricornutum | 2798 | 219127009 | Bacillariophyta | Eukaryota | 4 |
| Mch1 | Microcoleus chthonoplastes | 267 | 224407624 | Cyanobacteria | Bacteria | 8 |
| Ter2 | Trichodesmium erythraeum | 305 | 113475233 | Cyanobacteria | Bacteria | 8 |
| Ssp1 | Synechococcus sp. JA-3-3Ab | 317 | 86606127 | Cyanobacteria | Bacteria | 8 |
| Pca1 | Pyrobaculum calidifontis JCM 11548 | 244 | 126458964 | Crenarchaeota | Archaea | 8 |
| Average Size= 561 +/− 839 (all) | ||||||
| Average Size= 282 +/− 25 (w/out Ptr3) | ||||||
| Cluster 6 | ||||||
| Sus1 | Candidatus Solibacter usitatus Ellin6076 | 281 | 116624708 | Acidobacteria | Bacteria | 8 |
| Sth2 | Symbiobacterium thermophilum IAM 14863 | 279 | 51892120 | Firmicutes | Bacteria | 8 |
| Bsu1 | Brucella suis 1330 | 289 | 23500891 | Alphaproteobacteria | Bacteria | 8 |
| Ooe1 | Oenococcus oeni PSU-1 | 283 | 116491798 | Firmicutes | Bacteria | 8 |
| Pto1 | Picrophilus torridus DSM 9790 | 333 | 48478318 | Euryarchaeota | Archaea | 8 |
| Sth1 | Sphaerobacter thermophilus | 282 | 229877687 | Chloroflexi | Bacteria | 8 |
| Mth2 | Moorella thermoacetica | 271 | 83589239 | Firmicutes | Bacteria | 8 |
| Kcr1 | Candidatus Korarchaeum cryptofilum OPF8 | 285 | 170290371 | Korarchaeota | Archaea | 8 |
| Mxa1 | Myxococcus xanthus DK 1622 | 260 | 108758495 | Deltaproteobacteria | Bacteria | 7 |
| Dra1 | Deinococcus radiodurans R1 | 255 | 15805571 | Deinococcus-Thermus | Bacteria | 8 |
| Sma1 | Staphylothermus marinus F1 | 250 | 126465319 | Crenarchaeota | Archaea | 8 |
| Dac1 | Denitrovibrio acetiphilus | 274 | 227423788 | Deferribacteres | Bacteria | 7 |
| Emi1 | Elusimicrobium minutum Pei191 | 275 | 187251557 | candidate division TG1 | Bacteria | 7 |
| Hbu2 | Hyperthermus butylicus | 255 | 124028506 | Crenarchaeota | Archaea | 8 |
| Average Size= 277 +/− 20 (all) | ||||||
| Cluster 7 | ||||||
| Bad1 | Bifidobacterium adolescentis ATCC 15703 | 292 | 119026567 | Actinobacteria | Bacteria | 8 |
| Gva1 | Gardnerella vaginalis ATCC 14019 | 267 | 227507357 | Actinobacteria | Bacteria | 8 |
| Average Size= 280 +/− 18 (all) | ||||||
| Cluster 8 | ||||||
| Asa1 | Aliivibrio salmonicida | 279 | 16605593 | Gammaproteobacteria | Bacteria | 8 |
| Rru1 | Rhodospirillum rubrum | 276 | 83592684 | Alphaproteobacteria | Bacteria | 8 |
| Rsp3 | Roseovarius sp. HTCC2601 | 274 | 114764120 | Alphaproteobacteria | Bacteria | 8 |
| Rsp1 | Ruegeria sp. TM1040 | 278 | 99080207 | Alphaproteobacteria | Bacteria | 7 |
| Msp3 | Magnetococcus sp. MC-1 | 265 | 117925601 | Proteobacteria | Bacteria | 6 |
| Fpe1 | Fulvimarina pelagi HTCC2506 | 275 | 114707272 | Alphaproteobacteria | Bacteria | 7 |
| Bja2 | Bradyrhizobium japonicum USDA 110 | 287 | 27375621 | Alphaproteobacteria | Bacteria | 6 |
| Hne1 | Hyphomonas neptunium ATCC 15444 | 314 | 114797241 | Alphaproteobacteria | Bacteria | 9 |
| Cbu2 | Coxiella burnetii RSA 331 | 275 | 161831015 | Gammaproteobacteria | Bacteria | 7 |
| Lsp1 | Limnobacter sp. MED105 | 278 | 149925520 | Betaproteobacteria | Bacteria | 7 |
| Nmo1 | Nitrococcus mobilis Nb-231 | 266 | 88811005 | Gammaproteobacteria | Bacteria | 8 |
| Mca1 | Methylococcus capsulatus str. Bath | 294 | 53802665 | Gammaproteobacteria | Bacteria | 6 |
| Pir1 | Polaribacter irgensii 23-P | 281 | 88803086 | Bacteroidetes | Bacteria | 7 |
| Kko1 | Kangiella koreensis DSM 16069 | 268 | 227997603 | Gammaproteobacteria | Bacteria | 8 |
| Har1 | Herminiimonas arsenicoxydans | 287 | 134096092 | Betaproteobacteria | Bacteria | 7 |
| Swo1 | Shewanella woodyi ATCC 51908 | 268 | 170728324 | Gammaproteobacteria | Bacteria | 8 |
| Ptu1 | Pseudoalteromonas tunicata D2 | 269 | 88860323 | Gammaproteobacteria | Bacteria | 8 |
| Ama2 | Alteromonas macleodii 'Deep ecotype' | 268 | 196158505 | Gammaproteobacteria | Bacteria | 7 |
| Sbe1 | Shewanella benthica KT99 | 267 | 163752420 | Gammaproteobacteria | Bacteria | 8 |
| Msu1 | Mannheimia succiniciproducens MBEL55E | 266 | 52424462 | Gammaproteobacteria | Bacteria | 8 |
| Psp3 | Photobacterium sp. SKA34 | 267 | 89072545 | Gammaproteobacteria | Bacteria | 8 |
| Afe1 | Acidithiobacillus ferrooxidans ATCC 23270 | 264 | 218665563 | Gammaproteobacteria | Bacteria | 8 |
| Pne1 | Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1 | 272 | 145589361 | Betaproteobacteria | Bacteria | 8 |
| Lsp2 | Limnobacter sp. MED105 | 289 | 149926219 | Betaproteobacteria | Bacteria | 8 |
| Eco1 | Eikenella corrodens ATCC 23834 | 270 | 225024689 | Betaproteobacteria | Bacteria | 7 |
| Ama1 | Acaryochloris marina | 278 | 158336922 | Cyanobacteria | Bacteria | 9 |
| Tsp1 | Thioalkalivibrio sp. K90mix | 268 | 224818668 | Gammaproteobacteria | Bacteria | 8 |
| Rso1 | Ralstonia solanacearum | 273 | 17549483 | Betaproteobacteria | Bacteria | 8 |
| Ppe1 | Proteus penneri ATCC 35198 | 271 | 226330327 | Gammaproteobacteria | Bacteria | 8 |
| Iba1 | Idiomarina baltica OS145 | 264 | 85713215 | Gammaproteobacteria | Bacteria | 8 |
| Average Size= 275 +/− 11 (all) | ||||||
| Cluster 9 | ||||||
| Epe1 | Endoriftia persephone 'Hot96_1+Hot96_2' | 287 | 167948520 | Gammaproteobacteria | Bacteria | 5 |
| Cluster 10 | ||||||
| Pal1 | Providencia alcalifaciens DSM 30120 | 271 | 212712467 | Gammaproteobacteria | Bacteria | 8 |
| Cluster 11 | ||||||
| Orf3 | uncultured archaeon GZfos34A6 | 276 | 52549977 | none | Archaea | 8 |
| Mma1 | Methanosarcina mazei Go1 | 270 | 21228951 | Euryarchaeota | Archaea | 8 |
| Mma2 | Methanococcus maripaludis S2 | 270 | 45358505 | Euryarchaeota | Archaea | 8 |
| Average Size= 272 +/− 3 (all) | ||||||
| Cluster12 | ||||||
| Tko1 | Thermococcus kodakarensis KOD1 | 254 | 57640914 | Euryarchaeota | Archaea | 7 |
| Tba1 | Thermococcus barophilus MP | 251 | 223475524 | Euryarchaeota | Archaea | 8 |
| Mbo1 | Methanoregula boonei 6A8 | 269 | 154149849 | Euryarchaeota | Archaea | 8 |
| Sma2 | Staphylothermus marinus F1 | 265 | 126466107 | Crenarchaeota | Archaea | 8 |
| Average Size= 260 +/− 9 (all) | ||||||
| Cluster 13 | ||||||
| Tte1 | Thermobaculum terrenum ATCC BAA-798 | 255 | 227375491 | none | Bacteria | 8 |
| Cbe1 | Clostridium beijerinckii NCIMB 8052 | 272 | 150017843 | Firmicutes | Bacteria | 8 |
| Vdi1 | Veillonella dispar ATCC 17748 | 264 | 238018311 | Firmicutes | Bacteria | 8 |
| Nma1 | Nitrosopumilus maritimus SCM1 | 257 | 161528556 | Crenarchaeota | Archaea | 7 |
| Bbr2 | Brevibacillus brevis | 274 | 226314422 | Firmicutes | Bacteria | 7 |
| Gka1 | Geobacillus kaustophilus HTA426 | 300 | 56421519 | Firmicutes | Bacteria | 8 |
| Bcl1 | Bacillus clausii KSM-K16 | 272 | 56964722 | Firmicutes | Bacteria | 8 |
| Psp2 | Paenibacillus sp. JDR-2 | 272 | 251794851 | Firmicutes | Bacteria | 8 |
| Oih1 | Oceanobacillus iheyensis HTE831 | 285 | 23099829 | Firmicutes | Bacteria | 8 |
| Sau1 | Staphylococcus aureus subsp. aureus Mu50 | 275 | 15923912 | Firmicutes | Bacteria | 8 |
| Average Size= 273 +/− 13 (all) | ||||||
| Cluster 14 | ||||||
| Bja1 | Bradyrhizobium japonicum USDA 110 | 380 | 27376265 | Alphaproteobacteria | Bacteria | 8 |
| Ssp5 | Sphingomonas sp. SKA58 | 304 | 94498747 | Alphaproteobacteria | Bacteria | 7 |
| Pth1 | Pelotomaculum | 299 | 147678596 | Firmicutes | Bacteria | 7 |
| thermopropionicum SI | ||||||
| Dau1 | Desulforudis audaxviator | 394 | 169832116 | Firmicutes | Bacteria | 8 |
| Dre4 | Desulfotomaculum reducens MI-1 | 426 | 134299284 | Firmicutes | Bacteria | 9 |
| Abo1 | Aciduliprofundum boonei T469 | 254 | 223473124 | Euryarchaeota | Archaea | 8 |
| Lbi1 | Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)' | 325 | 183219704 | Spirochaetes | Bacteria | 8 |
| Average Size= 340 +/− 61 (all) | ||||||
| Average Size= 296 +/− 30 (w/out Bja1, Dau1, Dre4) | ||||||
| Cluster 15 | ||||||
| Orf1 | synthetic construct | 284 | 62258462 | none | Unclassified | 8 |
| Vsp2 | Verrucomicrobium spinosum DSM 4136 | 264 | 171915322 | Verrucomicrobia | Bacteria | 8 |
| Rme1 | Ralstonia metallidurans CH34 | 268 | 94311333 | Betaproteobacteria | Bacteria | 8 |
| Pre1 | Providencia rettgeri DSM 1131 | 264 | 223992411 | Gammaproteobacteria | Bacteria | 8 |
| Sso1 | Sulfolobus solfataricus P2 | 293 | 15899038 | Crenarchaeota | Archaea | 8 |
| Mmu1 | Mobiluncus mulieris 35243 | 361 | 227876711 | Actinobacteria | Bacteria | 9 |
| Aau1 | Arthrobacter aurescens TC1 | 300 | 119952309 | Actinobacteria | Bacteria | 8 |
| Lmo1 | Listeria monocytogenes EGD-e | 246 | 16802663 | Firmicutes | Bacteria | 8 |
| Ste2 | Sebaldella termitidis ATCC 33386 | 246 | 229881273 | Fusobacteria | Bacteria | 7 |
| Bsp2 | Bacillus sp. B14905 | 282 | 126650500 | Firmicutes | Bacteria | 8 |
| Cph2 | Chlorobium phaeobacteroides | 408 | 189499528 | Chlorobi | Bacteria | 9 |
| Afu1 | Archaeoglobus fulgidus | 325 | 11499708 | Euryarchaeota | Archaea | 7 |
| Dha1 | Desulfitobacterium hafniense DCB-2 | 312 | 219669180 | Firmicutes | Bacteria | 7 |
| Dre3 | Desulfohalobium retbaense | 569 | 227420936 | Deltaproteobacteria | Bacteria | 7 |
| Average Size= 316 +/− 86 (all) | ||||||
| Average Size= 280 +/− 26 (w/out Mmu1, Cph2, Dre3) | ||||||