Figure 1. Non-cell autonomous overgrowth of eye and head cuticle in mosaics with deregulated Hh signaling.

Anterior is to the left and images are from females. Mosaics were obtained by ey-FLP (eF) induced mitotic recombination, symbolized by >. MARCM-induced expression of transgenes such as Ci75 is symbolized by ::.

(A–H) Wild-type tissue is marked with red (w⁺) eye pigment while mutant tissue is white (w⁻). In wild type mosaics (A), the eye is a mixture of red and white eye tissue. In cos2 (B,C) and pka-C1 mosaics (D), not only are the eyes overgrown, but most of the pigment is red, i.e. cos2⁺ and pka-C1⁺, indicating that mostly wild type tissue contributes to the overgrown tissue. The overgrowth is most apparent at the anterior margin especially at the midline (arrows) of the eye (see also K). Some white tissue is apparent in the eye as well, indicating that the clones can survive, but have a growth disadvantage. Compared to controls (E), head cuticle also overgrows in cos2 (F) and ptc (G) mosaics. Reduced Hh signaling in smo mosaics (H) results in eyes of reduced size.

(I–L) Expression of Ci75 in cos2 mutant clones using the MARCM system suppresses the overgrowth in cos2 mosaics (K,L). Ectopic expression of Ci75 by itself in wild-type clones does not alter the size of the eye (J).

Genotypes:

1. y w ey-FLP; FRT42D P[w⁺] / FRT42D.
2. y w ey-FLP; FRT42D P[w⁺] / FRT42D cos2^P50.
3. y w ey-FLP; FRT42D P[w⁺] / FRT42D ptc^S2.
4. y w ey-FLP; P[w⁺] FRT40 / P[y⁺] pka-C1^K2 FRT40.
5. y w ey-FLP; FRT42D P[w⁺] / CyO.
6. y w ey-FLP; FRT42D P[w⁺] / FRT42D cos2^H29.
7. y w ey-FLP; FRT42D P[w⁺] / FRT42D ptc^S2.
8. y w ey-FLP; P[w⁺] FRT40 / P[y⁺] smo³ FRT40.
9. y w hs-FLP; FRT42 P[tubP-GAL80]/FRT42; P[tubP-GAL4]/+.
10. y w hs-FLP; FRT42 P[tubP-GAL80]/FRT42; P[tubP-GAL4]/UAS-Ci⁷⁵.
11. y w hs-FLP; FRT42 P[tubP-GAL80]/FRT42 cos2^H29 ; P[tubP-GAL4]/+.
12. y w hs-FLP; FRT42 P[tubP-GAL80]/FRT42 cos2^H29 ; P[tubP-GAL4]/UAS-Ci⁷⁵.