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. Author manuscript; available in PMC: 2014 Feb 7.
Published in final edited form as: Mech Dev. 2012 Jun 5;129(0):98–108. doi: 10.1016/j.mod.2012.05.009

Figure 3. Neither cos2 nor ptc mutant tissue is sufficient for overgrowth.

Figure 3

(A–D) Shown are fly eyes obtained by the cell-lethal method. (A) In the unflipped control, the GMR-hid transgene ablates most heterozygous cells and provides red eye pigment. (B) In trans to a wild type chromosome, the twin spot cells survive and re-populate the eye with a few red surviving cells from the dominant marker (GMR-hid). (C,D) Neither cos2 nor ptc mutant tissue generate a full sized adult eye. Especially ptc mutant eyes are significantly reduced in size (D).

(E–G) Anti-Hid antibody labelings of control (E), cos2 (F) and ptc (G) mosaic eye discs obtained by ey-FLP-induced mitotic recombination. In cos2 and ptc mutant clones posterior to the MF, Hid levels are elevated (arrows).

Genotypes:
  • (A) y w; FRT42 GMR-hid l(2)CL-R/+; ey-GAL4 UAS-FLP
  • (B) y w; FRT42 GMR-hid l(2)CL-R/FRT42; ey-GAL4 UAS-FLP
  • (C) y w; FRT42 GMR-hid l(2)CL-R/FRT42 cos2H29 y+; ey-GAL4 UAS-FLP
  • (D) y w; FRT42 GMR-hid l(2)CL-R/FRT42 ptcC y+; ey-GAL4 UAS-FLP
  • (E) y w ey-FLP; FRT42 ubi-GFP/CyO
  • (F) y w ey-FLP; FRT42 cos2H29 /FRT42 ubi-GFP
  • (G) y w ey-FLP; FRT42 ptcC /FRT42 ubi-GFP