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. Author manuscript; available in PMC: 2014 Feb 7.
Published in final edited form as: Mech Dev. 2012 Jun 5;129(0):98–108. doi: 10.1016/j.mod.2012.05.009

Figure 4. cos2 mutants generate precocious, autonomous and non-autonomous differentiation in the eye imaginal disc.

Figure 4

(A) ELAV marks the differentiating neuronal tissue posterior to the MF in control discs.

(B) In ey-FLP-induced cos2 mosaics, ELAV expression does extend anterior to the MF. This precocious expansion is associated with mutant clones, but is also seen in adjacent cos2+ tissue (shown in inset outlined in dashed line). ELAV-labeling is not grossly altered in cos2 clones posterior to the MF.

(C) dpp-lacZ marks the MF in wild-type eye discs.

(D) In ey-FLP-induced cos2 mosaics, multiple changes in dpp-lacZ expression are observed. The MF is shifted anterior around clones crossing the MF (arrow in D’). These clones reduce expression of dpp-lacZ (arrowhead). In contrast, clones further anterior and not associated with the MF autonomously induce dpp-lacZ expression (asterisk). β-GAL staining posterior to the MF is due to a weak GMR-lacZ reporter on the ey-FLP chromosome.

Genotypes:
  • (A) y w ey-FLP; FRT42 ubi-GFP/CyO
  • (B) y w ey-FLP; FRT42 cos2H29 /FRT42 ubi-GFP
  • (C) y w ey-FLP; ubi-GFP FRT40/CyO; dpp-lacZ
  • (D) y w ey-FLP GMR-lacZ; FRT42 cos2H29 /FRT42 ubi-GFP; dpp-lacZ