Table 1.
Characteristics of the A. cellulolyticus strains and plasmids used in this study
| Strain or Plasmid | Description | References |
|---|---|---|
| Strain |
|
|
|
A. cellulolyticus Y-94 |
Wild type (FERM BP-5826) |
Yamanobe et al. (1987) |
| YP-4 |
Uracil auxotroph mutant derived from Y-94 |
Inoue et al. (2013) |
| YDCre |
YP-4 prototrophic transformant horboring pDCre2500, creA disruptant. |
This study |
| YPyrF |
YP-4 prototrophic transformant horboring a single copy pbs-pyrF in pyrF loci. |
This study |
| YCreGFP |
YP-4 prototrophic transformant harboring pCreGFP |
This study |
| Plasmid |
|
|
| pbs-pyrF |
Ampr PyrFr; pBluescript KS(+) derivative containing 2.7-kb fragment harboring pyrF from Y-94 |
Fujii et al. (2012) |
| pDCre2500 |
Ampr PyrFr; pbs-pyrF derivative containing 2.5 kb upstream and 2.5 kb downstream regions of creA |
This study |
| pDCre1000 |
Ampr PyrFr; pbs-pyrF derivative containing 1.0 kb upstream and 1.0 kb downstream regions of creA |
This study |
| pCreGFP | Ampr PyrFr; pbs-pyrF derivative containing GFP and creA fused gene | This study |