Table I.
Primers for quantitative polymerase chain reaction.
| Primer name | Sequence | Base pairs | Temperature (°C) | Cycles | |
|---|---|---|---|---|---|
| COL21A1 | sense | 5′-GGATTAATGGGTAGTCCCGGTTTC-3′ | 149 | 60 | 45 |
| antisense | 5′-TGTCCTGGAGGCCCAATTTC-3′ | ||||
| COL22A1 | sense | 5′-GTGATTGGCAAGCGCCTCTAC-3′ | 98 | 60 | 45 |
| antisense | 5′-CAAGTCTCCAATTCTGCGTGTCTC-3′ | ||||
| COL23A1 | sense | 5′-AAGCTCCATCCGAATGTGTCTG-3′ | 108 | 60 | 45 |
| antisense | 5′-GGTAGCCATCTCGTCCTGATTG-3′ | ||||
| COL24A1 | sense | 5′-CCCAGCACGAATCTGCAAAG-3′ | 135 | 60 | 45 |
| antisense | 5′-GTCTGGCCACCAGCACTGAA-3′ | ||||
| GAPDH | sense | 5′-GCACCGTCAAGGCTGAGAAC-3′ | 138 | 60 | 40 |
| antisense | 5′-TGGTGAAGACGCCAGTGGA-3′ | ||||