Figure 5. flaA:Ova mediated IL-6 secretion depends on both proteasome and inflammasome activation.

BALB/c mDC were stimulated with the indicated equimolar amounts of rflaA, rflaA+rOva and rflaA:Ova for 24 h and the resulting IL-6 and IL-10 secretion in the culture supernatants was analyzed by ELISA (A). BALB/c mDC were incubated with either the inflammasome inhibitors Z-VAD-FMK and glyburide (or the proteasome inhibitor lactacystin) for 2 h (B). Subsequently mDC were stimulated for 22 h with 4 µg of rflaA:Ova. Supernatants were analyzed for IL-6 secretion via ELISA. Results are mean values of three independent experiments ± SD.