Table 1. Data collection and structure refinement statistics.
| Data collection | LASV L endonuclease with two Mg2+ ions |
| Wavelength (Å) | 0.9793 |
| Space group | P43212 |
| Unit-cell parameters (Å; °) | 57.72, 57.72, 134.51; 90, 90, 90 |
| Resolution (Å) | 40.81–1.72 (1.85–1.72)a |
| Unique reflections | 24168 (2208) |
| Completeness (%) | 96.65 (90.71) |
| Multiplicity | 9.3 (9.7) |
| Mean I/σ (I) | 43.65 (6.11) |
| Rmerge (%) | 0.048 (0.579) |
| Refinement | |
| Rfactor b | 16.51 (19.8) |
| Rfree c | 17.72 (25.43) |
| Number of atoms | 1546 |
| Protein | 1386 |
| Metal ions | 2 |
| Solvent | 158 |
| RMSD bond (Å)/angles (°) | 0.010/1.12 |
| Ramachandran favored (%) | 99% |
| Mean B-factors (Å2) | 22.9 |
| Protein | 21.9 |
| Solvent | 31.6 |
| PDB accession code | 4MIW |
a
Values in parentheses are for the highest resolution shell.
b
Rfactor = Σ||Fo|−|Fc||/Σ|Fo|, where Fo and Fc are observed and calculated as structure factors, respectively.
c
Rfree is calculated using 5% of total reflections, which is randomly selected as a free group and not used in refinement.