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. 2014 Feb 7;9(2):e88117. doi: 10.1371/journal.pone.0088117

Figure 4. Figure 4a. Parasite liver load is not significantly different between CPS and CPS-bs groups.

Figure 4

Animals (n = 3) were subjected to CPS (CPS) or CPS under patency (CPS-bs) as described and subsequently challenged with 10,000 NK65 sporozoites at day 70 post-first-immunization. Mice were sacrificed after 46–48 hours and parasite liver burden was quantified by quantitative real-time PCR analysis of parasitic 18S rRNA expression and normalized to infected but unvaccinated control mice. Figure 4b . Both normal CPS and CPS-bs induce increased CD8 expression in the liver. Animals (n = 4) were subjected to CPS (CPS) and CPS under patency (CPS-bs) as described and subsequently challenged with 10,000 NK65 sporozoites at day 70 post-first-immunization. Unvaccinated control mice (Control) were infected with 10,000 NK65 sporozoites. Mice were sacrificed after 46–48 hours and expression of CD8 was quantified by quantitative real-time PCR analysis.