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. 2014 Feb 7;9(2):e88179. doi: 10.1371/journal.pone.0088179

Figure 1. Workflow of the contig-based strategy with current mainstream method for miRNA identification.

Figure 1

Acquiring candidate miRNA precursors for hairpin structures is the first step in miRNA identification. This strategy is based on contigs from genomic DNA sequencing, replacing available dataset-based methods (represented by EST+GSS). Blue-flow. Pipeline of DNA sequencing [It is the innovation of this strategy]; Red-flow. Pipeline of small RNA sequencing.