Figure 1. Workflow of the contig-based strategy with current mainstream method for miRNA identification.
Acquiring candidate miRNA precursors for hairpin structures is the first step in miRNA identification. This strategy is based on contigs from genomic DNA sequencing, replacing available dataset-based methods (represented by EST+GSS). Blue-flow. Pipeline of DNA sequencing [It is the innovation of this strategy]; Red-flow. Pipeline of small RNA sequencing.