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. 2014 Feb 7;9(2):e88501. doi: 10.1371/journal.pone.0088501

Figure 6. Decreased pulmonary cell death in septic mice treated with cyclophosphamide or anti-CD18 antibodies is due to a specific decrease in pulmonary MVEC death.

Figure 6

(A) Endothelial cells were labeled with CD31 (left column of panels; green) and non-viable cells were stained with PI (red; middle column of panels). Overlap between cells positive for CD31 and PI (yellow) specifically identifies non-viable endothelial cells (right column of panels). (B) Quantification of the percentage of CD31-positive cells that are also PI-positive illustrates that treatment of septic mice with cyclophosphamide or anti-CD18 antibodies decreases the percentage of CD31-positive cells that are also PI-positive compared to untreated septic mice (*, p<0.05 for Untreated vs. other treatment groups; #, p<0.05 for anti-CD18 antibody treated vs. IgG control antibody treated; One-Way ANOVA with Tukey post-hoc test; n = 3–6 per treatment).