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. 2014 Feb 7;9(2):e88420. doi: 10.1371/journal.pone.0088420

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© 2014 Patarroyo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) IFA assay showing characteristic PfEMP1 dotted pattern on IE membrane, using sera from immunised Aotus monkeys, corresponding mHABP number on top. (B) WB recognition of ∼300 kDa protein in IE lysate from mHABP-immunised Aotus sera. PI: preimmune sera; NP: non-protected. (C) Comparative course of parasitaemia in Aotus immunised with mHABPs. Note the complete absence of parasites (full protective immunity) induced by 24196 (6510) in the first trial; the second with 10 monkeys gave similar results.