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. 2014 Feb 7;9(2):e88417. doi: 10.1371/journal.pone.0088417

Figure 1. Id1 and 3 are co-localized in kidney endothelial cells and expression levels are transiently increased following ischemia-reperfusion injury (IRI).

Figure 1

Immunofluorescence images for indicated antigens of normal (A–E) and 3 days post ischemia-reperfusion injury (F) kidneys from Id1+/+, Id3RFP/+ (C–F) and wild-type (WT) (A–B) mice. Red fluorescent protein (RFP) signal corresponds with Id3 expression. A) Green: Id1, red: CD31, arrows: Id1 negative, CD31 positive cells, arrowheads: Id1 low, CD 31 positive cells, B) Green: Id1, red: PDGFRβ, arrows: Id1 negative, PDGFRβ positive cells, C) Green: CD31, red: Id3RFP, arrows: double positive cells, D) Green: PDGFRβ, red: Id3RFP, arrows: single PDGFRβ positive cells, arrowheads: double positive mesangial cells, E) Green: Id1, red: Id3RFP, yellow: double positive cells in glomeruli (arrow) and interstitium (arrowhead), F) Green: Id1, red: Id3RFP, yellow: double positive cells in interstitium (arrows) 3 days following IRI, Original magnification: 400X. Representative Western blots of Id1 (G) and Id3 (H) expression following ischemia-reperfusion at indicated time points with corresponding densitometry from 5 mice for each time point. *p<.05 (unpaired two-tailed t-test).