Figure 4. (A) The effects of PKC-λ-specific inhibitor, ICAPP, on insulin-stimulated phosphorylation/activation of ERK in adipocytes of wild-type (WT) mice and (B) the effects of adipocyte-specific knockout (KO) of PKC-λ on ERK activity and levels in adipocytes of WT and KO mice. Mice in these studies were 5–7 mo old. In (A), adipocytes were isolated from ad lib fed WT mice and incubated for 30 min with indicated concentrations of PKC-λ inhibitor ICAPP, and then for 10 min ± indicated concentrations of insulin. In (B), adipocytes were isolated from ad lib fed WT and KO mice.