Figure 3. Altering callose deposition in developing lateral roots regulates branching density and percentage of emergence. Callose (white, stained with aniline blue) failed to deposit around stage IV–V primordia (LRP) treated for 48h with 50uM DDG in comparison to control (untreated) roots (A-B). Scale bars: 20 µm. Branching density (C) and percentage of emergence (D) were calculated in 9-d-old wild-type roots, untreated (Control) and treated with 50uM DDG for 72h, and in PDCB1 OE plants. Error bars represent SD. Statistical significance are represented as *, P < 0.1; **, P < 0.01; ***, P < 0.001.