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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Oncogene. 2013 May 20;33(18):2307–2316. doi: 10.1038/onc.2013.187

Figure 3. Calcium regulates the induction of some genes implicated in EGF-induced EMT.

Figure 3

Analysis of mRNA levels of EMT-associated genes (vimentin, Twist, N-cadherin, Snail and CD44/CD24) in MDA-MB-468 breast cancer cells with intracellular calcium chelation (BAPTA-AM). Cells were treated with EGF for 6 h (A, C, E, G and I) or 24 h (B, D, F, H and J) to induce EMT. Bar graphs show mean ± S.D. for nine individual wells from three independent experiments. The effect of BAPTA-AM on gene expression was assessed using two-way ANOVA with Bonferroni’s multiple comparisons post-tests. * P < 0.05.