Figure 3. DsrA·rpoS duplex formation in the presence of HfqG29A does not result in efficient translation of rpoS mRNA at low temperature. (A) Immunodetection of RpoS, ribosomal protein L9, and Hfq in strains JW4130(pAHfq) (lane 1), JW4130(pAHfqG29A) (lane 2), and JW4130(pACYC184) (lane 3). DsrA and 5S rRNA were detected as described in the legend to Fig. 1. Only the relevant parts of the immunoblots and autoradiographs are shown. (B) Lanes 1–3, primer extension analysis of total RNA isolated from strains JW4130(pAHfq), JW4130(pAHfqG29A), and JW4130(pACYC184), respectively. The primer extension (PE) signals for rpoS mRNA isolated from the different strains are shown on top. The RNase III-mediated cleavage signals in rpoS mRNA are marked by arrows (G-112 and A-109). G, G sequencing ladder.