Figure 3. aae-miR-981 downregulates importin β-4. (A) Western blot analyses showing levels of importin β-4 protein using a specific monoclonal antiserum to the protein in cytoplasmic (C) and nuclear (N) fractions of -Wol and +Wol cells, and (B) in Dicer-1 silenced cells along with gfp control. (C) RT-qPCR analysis shows significant (*P < 0.05) downregulation of importin β-4 transcript in aae-miR-981 mimic-transfected cells (mimic) as compared with the control mimic (C mimic). Error bars represent standard errors of averages from three biological replicates. (D) AGO1 protein levels in cytoplasmic and nuclear fractions of -Wol cells in control mimic and aae-miR-981 mimic transfected cells. (B and C) were re-probed with anti-hsp70 antibody to show equal loading of the samples. (A and D) were re-probed with anti-GAPDH antibody to show equal loading of samples. Graphs indicate normalization of proteins to GAPDH and Histone H3 using imageJ software.