Figure 5. Prohibitin associates with AGO1. (A) Immunoprecipitation of AGO1 in -Wol and +Wol cells. A 50 kDa protein (arrow) was found in considerably higher quantities in +Wol cells and a 72 kDa (arrowhead) protein in much less quantities in +Wol cells. (B) Western blot analysis of -Wol and +Wol cells to detect prohibitin. (C) AGO1 protein levels in mock, GFP and prohibitin dsRNA-transfected +Wol cells; hsp70 shows equal loading. (D) Northern blot hybridization with three different miRNA probes in mock, prohibitin RNAi, and GFP RNAi in +Wol cells. The same blot was washed with 0.1% boiling SDS for stripping and reused for other probes. U6 shows equal loading.