Fig 5.

Immunohistochemical analysis of reconstructed lung tissue sheets in vitro. (A, D) Pan-CK-positive cells were concentrated as a monolayer around of the alveolus-like structures in sheets after 7 and 14 days of culture. (B, E) Vimentin positive cells were distributed in reconstructed tissue sheet after 7 and 14 days of culture. (C, F) SpC-positive cells were detected in sheets after 7 and 14 days of culture. (G, I) Pan-CK-positive cells and SpC-positive cells decreased in 21-day-cultured lung tissue sheets. (H) There were no vimentin-positive results in 21-day-cultured lung tissue sheets. Bars = 100 μm.