Fig 2.

MiR-107 promotes gastric cancer cell invasion and metastasis in vitro and in vivo. (A) The expression level of mature miR-107 in various gastric cancer cell lines was determined using quantitative PCR. (B) MiR-107 sponge inhibition function was assayed in SGC-7901 or MKN-45 cells. Luciferase reporter assays were used to examine the sponge inhibition efficiency. (C, D) Transwell migration assay (C) and Matrigel invasion assay (D) of SGC-7901 or MKN-45 cells transfected with miR-107 sponge or its control retrovirus. Magnification in D2, 200×. (E) Mice were injected with 1×10⁶ cells through tail vein. Four weeks later, the mice were sacrificed. The liver tissues were observed with naked eyes and the number of visible tumours in liver surface was counted (E1). The liver tissues were made serial sections before being haematoxylin and eosin dyed and observed under a light microscope (E2). N = 3; error bars represent S.E.M. P-values obtained using a one-sided Student’s t-test. P < 0.05 versus cells transfected with control retrovirus.