In vivo engraftment of GFP transduced SS-AF-MPCs. (A) GFP-SS-AF-MPCs were trapped in different tissues as evaluated by immunohistochemistry, 4 and 10 days after transplantation. GFP-SS-AF-MPCs were found in liver, spleen, lung and kidney at low frequency. Immunohistochemistry was performed by using anti-GFP antibody. Quantitation of GFP cells was determined by (B) FACS analysis and (C) RT-PCR in the respective tissues 4 or 10 days post-injection where percentage of positive cells and GFP fold expression difference is presented, respectively. As negative controls, non-injected mice were used. Values are shown as mean ± S.D. for four mice in each group. (D) (i) Representative post-mortem fluorescent microscopy image of SS-AF-MPCs within the matrigel revealed a robust engraftment. (ii) FACS analysis of the disassociated matrigel area in presence of GFP transduced SS-AF-MPCs (red filled histogram) or GFP transduced SS-AF-MPCs in PBS (open histogram) 1 and 10 days post-transplantation.