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Inhibition of cardiac EpoR mRNA expression in transgenic mice with short hairpin RNA against Gata4. Transgenic mice with a stably integrated, doxycycline-inducible Gata4 shRNA construct and FvB wild-type mice (control) were fed with 20 mg doxycycline per ml drinking water over 38 days. Endogenous Gata4 and EpoR expression levels were quantified by Q-PCR and normalized to β-actin. All samples were measured in duplicates according to the threshold cycle method (**P < 0.01; *P < 0.05).
