Fig 1.

Characterization of freshly isolated BM-CD105⁺cells and cultured MSCs. (A) Morphology of immunomagnetically isolated BM-CD105⁺ cells after they were cytocentrifuged and counterstained with May-Grünwald solution. (B) Three colour flow cytometric analysis of sorted cells demonstrated two distinct CD105⁺ cell populations: CD105⁺Glycophorin-A⁺CD45⁻ and CD105⁺Glycophorin-A⁻CD45⁻. (C–D) Fibroblastoid morphology of cells derived from CD105⁺ cells after 3 (C) and 10 days (D) in culture before first passage (primary cells).